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in the embodiment, a Tet repressor is preferably mutated at least one of these positions? The term “modified tetracycline repressor” or “modified revTetR” further include Tet repressors wherein the amino-terminal DNA-binding domain is derived from a DNA-binding protein other than a TetR repressor protein, and the DNA sequence to which such a chimeric tetracycline repressor protein binds corresponds to the DNA sequence recognized and bound by the non-TetR repressor, DNA-binding protein.